study the effect of methyl jasmonate and salicylic acid on ‎morphological characters internal pigments of artichoke callus

modern biotechnology and genetic engineering using techniques and special ‎laboratory tools plays an important role in the pharmaceutical industry and medicinal ‎plant propagation. among these techniques, it can be noted that a wide range of ‎elicitors are widely used for inducing metabolites at invitro culture. due to the ‎pharmaceutical properties of artichoke and its history, it has been deeply noticed by ‎pharmaceutical industry and its cultivation is expanding rapidly. in present study the ‎effect of two elicitors' methyl jasmonate (mj) and salicylic acid (sa) on the callus ‎mass growth and survival of invitro culture was investigated. callus cultures of ‎artichoke were established by transferring seedling on solidified ms medium ‎supplemented with different concentrations of salicylic acid and methyl jasmonate. ‎four weeks after subculture, the callus was collected and weighed. results showed ‎that the sa supplementation reduced fresh weight of callus. contrary to fresh weight, ‎a direct increase was observed in dray weight by increasing the level of sa. opposite ‎to sa different concentration of mj not only did not changed the fresh weight, but ‎also reduced the dry weight of callus. study the pigment changes under different ‎treatments of sa and mj showed that, contrary to cartonoid a direct reduction was ‎observed in chlorophyll content by increasing in both sa and mj concentration. ‎finally it can be suggested that, however both sa and mj as most important elicitors ‎increase secondary metabolite production of callus, their availability in high ‎concentration affect the cell grow activity, callus survival and the biomass production.‎